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Fig. 9. Activation of Smad2 inhibits neural induction in Xenopus
explants. (A) Activation of Smad2 by DEX at blastula to early
gastrula stages suppressed neural induction by noggin and induced mesodermal
markers in animal caps; but it could not do so efficiently if activated at
mid- to late gastrula stages. noggin (10 pg) and GR-Smad2 (0.2 ng) RNAs were
used. (B) Activin protein (1:50 dilution of oocyte conditioned medium)
reduced neural and induced mesodermal markers in dorsal ectoderm explanted
from stages 11-11.5 embryos, but not from stage 12 embryos. (C) Unlike
dorsal ectoderm, ventral ectoderm did not respond to activin efficiently at
mid-gastrula stages (stage 11 onward).