DEV009597 Supplementary Material

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• Supplemental Table S1 -

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• Supplemental Figure S1 -

  Fig. S1. Promoter swap assays. (A) Expression of HA-TCL1 under the control of the TRY promoter in a try mutant background represses trichome initiation on leaves (top) but has no effect on root hair initiation (bottom). Arrows indicate that trichome clusters are still present on the leaves of the P_TCL1:HA-TCL1 transformed try mutant. (B) Expression of HA-TCL1 under the control of the CPC promoter in a cpc mutant background represses trichome initiation on leaves (top) and partially rescues the root hair phenotype of the cpc mutant (bottom). (C) Expression of HA-TCL1 in try and cpc mutants examined by RT-PCR using a pair of primers − one HA-specific, the other TCL1-specific. Expression of ACTIN2 provided a control.

• Supplemental Figure S2 -

  Fig. S2. Expression of TCL1. (A) Expression pattern of TCL1 in various tissues/organs. The expression pattern of TCL1 was examined using transgenic plants expressing a TCL1 promoter-GUS fusion construct (P_TCL1:GUS). Top left, expression of TCL1 in the cotyledons of a 7-day-old seedling; bottom left, developing leaves, showing strong staining in the trichome sites; bottom middle, root of a 7-day-old seedlings, showing staining in the vascular system but no staining in the root tip; top right, expanded leaves, showing the strong staining at the bases of trichomes; bottom right, inflorescence, showing staining in floral organs. (B) Expression of P_TCL1:GUS is developmentally regulated. Top, comparison of TCL1 expression in the lower part of the hypocotyl in 4-day-old (left) and 7-day-old (right) seedlings; middle and bottom, expression of TCL1 in emerging (middle left), emerged (middle right) and elongated (bottom) lateral roots.

• Supplemental Figure S3 -

  Fig. S3. RT-PCR analysis of GL1 transcript in seedlings overexpressing TRY or CPC. 35S:TRY is in the Landsberg erecta (Ler) ecotypic background. 35S:CPC is in the Wassilewskija (WS) ecotypic background. Expression of ACTIN2 provided a control.