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Fig. 5. Immuno-TEM detection of 3xMyc-Nodal at the node.
(A-D) Immunohistochemical staining for 3xMyc-Nodal in transverse
frozen sections of E8.0 mouse embryos positive (A-C) or negative (D) for the
transgene shown in Fig. 4A.
(E-H) Ultrathin sections corresponding to the boxed regions shown in A
to D, respectively, were examined by TEM at x1000. (I-M) The
boxed regions in E-G are shown at higher magnification (x4000) in I-K,
respectively. Black dots, cell boundaries; green dots delineate the nucleus.
The boxed regions in J and K are shown at even higher magnification
(x6000) in L and M, respectively, revealing apparent secretion of
3xMyc-Nodal at the basolateral membranes. Scale bars: 10 µm in A-H;
2.5 µm in I-K; 1 µm in L,M.
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