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Figure 2


Fig. 2. Loss of bro-1 function causes V- and T-lineage-specific division failures, resulting in ray loss. (A) him-8(e1489) male tail, with the characteristic nine rays per side (numbered). (B) rnt-1(ok351); him-8(e1489) male tail. Arrowheads indicate four rays visible on the left side. (C) bro-1(tm1183); him-8(e1489) male tail. Arrowheads indicate three visible rays. (D) Bar chart showing ray number in him-8(e1489) (n=102; effectively WT), bro-1(tm1183); him-8(e1489) (n=94) and bro-1(tm1183); him-8(e1489); msEx436[pHK196 + rol-6] (n=43) males. Error bars represent the standard error of the mean (s.e.m.). (E) Bar chart showing seam cell number in WT adult hermaphrodites carrying the seam cell marker SCM::GFP (strain JR667; n=70), bro-1(tm1183); SCM::GFP (n=83) and bro-1(tm1229); SCM::GFP (n=54) hermaphrodites. (F,G) Lineage trace of V1-V6 and T divisions in WT and bro-1 animals up to mid L3. The L1 asymmetric division is omitted for simplicity. Broken lines indicate incomplete lineages. Question mark indicates fate was not determined. The data shown are lineage traces for single animals but are representative of several lineaged animals. (Fi) WT male. (Gi) WT hermaphrodite. Divisions are as previously described (Sulston et al., 1980). F(ii): bro-1(tm1183) male. Several division failures are evident as shown. (Gii) bro-1(tm1183) hermaphrodite. A similar scale of defects is observed. (H) Lineage trace of the T divisions in WT and bro-1 animals during L1. (Hi) WT hermaphrodite. (Hii) bro-1(tm1183) hermaphrodite. The anterior branch of the T lineage looks normal, but the posterior branch fails. T.pa and T.pp have a hypodermal appearance and fail to divide further. (I-L) Nomarski and fluorescence (dpy-7::GFP) images of T-lineage cells in WT and bro-1 L1 animals. Asterisks indicate hypodermal nuclei. White arrowheads indicate T.px nuclei T.pa and T.pp and white arrows indicate T.ax hypodermal/seam nuclei. (I) WT L1 hermaphrodite. (J) bro-1(tm1183) L1 hermaphrodite. T.px nuclei look hypodermal in both strains. (K) WT L1 hermaphrodite carrying dpy-7::GFP. (L) bro-1(tm1183) L1 hermaphrodite carrying dpy-7::GFP (strain AW194). dpy-7::GFP expression is similar in all four nuclei in both strains.





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