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Figure 8


Fig. 8. Co-overexpression of bro-1 and rnt-1 induces massive seam cell hyperplasia. (A) Bar chart showing seam cell number in SCM::GFP him-5(e1490) L4 hermaphrodites (n=24; effectively WT), and animals with the same reporter carrying an integrated bro-1::GFP transgene (labelled as Is[BRO-1], strain AW188; n=57), an integrated rnt-1::GFP transgene (labelled as Is[RNT-1], strain AW189; n=55), and both integrated bro-1::GFP and rnt-1::GFP transgenes (labelled as Is[BRO-1];Is[RNT-1], strain AW190; n=38). (B) Seam cell nuclei along the mid body region of a WT L4 hermaphrodite animal carrying the SCM::GFP reporter. Scale bar: 25 µm. (C,D) Similar region of L4 hermaphrodites co-overexpressing bro-1::GFP and rnt-1::GFP as well as SCM::GFP (strain AW190). Scale bars: 25 µm. (E,F) Low power images showing whole worms. Scale bars: 100 µm. (E) WT adult hermaphrodite. (F) Adult hermaphrodite co-overexpressing bro-1::GFP and rnt-1::GFP.





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