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Figure 5


Fig. 5. Aberrant interzone formation in Prx1-Hif1{alpha} autopod. (A-D) In situ hybridizations for Gdf5. Whole-mount at E12.5 (A) and E13.5 (B) and sections at E13.5 (C) and E14.5 (D) show noticeable differences in the expression patterns between Prx1-Hif1{alpha} and control; white and black arrowheads indicate interzone area. (E) Noggin expression at E14.5. Red arrows indicate interzone area. (F-I) Higher magnification of the joint located between the first and the second phalanges of E15.5 control and Prx1-Hif1{alpha} autopods. (F) H&E staining. (G-I) Sections were hybridized with RNA probes for Gdf5 (G), Wnt9A (H) and Bmp2 (I).





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