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Fig. 5. Plcz1 expression in tumours and in the tumourigenic strain
LT/Sv, and expression of its human orthologue in tumours. (A)
Relative mRNA levels determined by qPCR for tumours from CV3 (stippled) and
CS16 (grey) relative to wild-type ovary, or combined and expressed relative to
skeletal muscle (white). Error bars, ±s.e.m.; *, not
detected. Corresponding P-values are shown for unpaired Student's
t-tests. (B) Tumour transcript levels of maternally- (white)
and paternally- (grey) expressed imprinted genes determined by qPCR and
represented relative to respective levels in wild-type ovary (1.0). Error
bars, ±s.e.m. P-values (above), unpaired Student's
t-tests. (C) RT-PCR analysis of two CV3 (CV) and four CS16
(CS) tumours, wild-type placenta (pl) and ovaries (o), for placental marker
and metastasis-associated gene expression. (D) RT-PCR (35 cycles) of
selected tissues from an 8-week-old LT/Sv female. Key as per
Fig. 1. (E) RT-PCR for
PLCZ1 and control ACTB mRNAs in ten independent human
ovarian germline tumour (hOGT) biopsy samples as indicated. t, mouse testis
control.
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