QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online October 12, 2007

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?

More to separase than chromosome separation

Fertilization triggers several events in oocytes, including resumption of the cell cycle and, in many organisms, exocytosis of cortical granules, the contents of which modify the extracellular covering of the zygote. Bembenek and co-workers now identify cortical granules in C. elegans for the first time and show that their exocytosis after fertilization is regulated by several cell-cycle components, most notably separase, which is required for chromosome segregation during anaphase (see p. 3837). The exocytosis of cortical granules in fertilized C. elegans oocytes, the researchers report, leads to the formation of an impermeable three-layered eggshell. Using RNAi knockdown, they show that separase is required for granule exocytosis and chromosome segregation. Then, using immunofluorescence and live-cell imaging, they show that, after fertilization, separase moves from filamentous structures into cortical granules. These, they report, are exocytosed during anaphase I. Together, these results lead the researchers to propose that separase helps to coordinate the cell cycle with the other events that occur during egg activation.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in Development:

Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase

Joshua N. Bembenek, Christopher T. Richie, Jayne M. Squirrell, Jay M. Campbell, Kevin W. Eliceiri, Dmitry Poteryaev, Anne Spang, Andy Golden, and John G. White
Development 2007 134: 3837-3848. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?