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Fig. 1. Different states of the skeletal muscle satellite cell. (A)
Pax7-positive satellite cells (red) at the surface of a single fiber isolated
from the extensor digitorum longus (EDL) muscle of an adult mouse. Nuclei are
stained with DAPI (blue). (B) During embryonic development, a
Pax7-positive population of myogenic progenitors contributes to myogenesis and
gives rise to satellite cells. Cryosection through a masseter muscle from an
E14 mouse embryo. Differentiated muscle fibers are stained with an antibody
against myosin heavy chain (cytoplasm, blue) and an antibody against myogenin
(nuclei, red). Pax7-positive cells (nuclei, green) are located between the
newly formed fibers. (C) Transverse cryosection of a transplanted mouse
tibialis muscle, showing that transplanted YFP-positive satellite cells can
fuse with host muscle fibers and contribute to the host stem cell niche.
Grafted cells are labeled with an anti-GFP antibody (green) and anti-Pax7
antibody (red). Muscle fiber basal laminas are labeled with a laminin antibody
(white) and nuclei with DAPI (blue). Arrow shows a grafted YFP-positive
Pax7-positive cell in a satellite cell position. Images in A and B are
courtesy of Fabien Le Grand; image in C is courtesy of S. Kuang.
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