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Fig. 3. Role of Hoxd12 in caecum agenesis. (A) Derivation of
the Del(4-11) allele by TAMERE. (B-J) Whole-mount RNA
in situ hybridization of Hoxd12 (B-D), Fgf10 (E-G) and
Pitx1 (H-J) transcripts in wild-type control (B,E,H),
Del(4-11)-deficient HoxD cluster mutant
heterozygous (C,F,I) and homozygous (D,G,J) posterior midguts at E12.5. In
wild-type controls, the Hoxd12 transcript is always absent from the
caecum bud (B), whereas localized expression of both Fgf10 (E) and
Pitx1 (H) are always detectable. Caecum bud growth is conspicuously
retarded in Del(4-11) heterozygous specimens and is
correlated with robust Hoxd12 transcript accumulation (C), reduced
hybridization signals for Fgf10 (F) and undetectable signal for
Pitx1 (I) in bud mesenchyme. Absence of bud growth in homozygous
specimens is correlated with extremely reduced of Fgf10 (asterisk in
G) and complete absence of localized Pitx1 (J).
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