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Files in this Data Supplement:
Fig. S1. Inner lateral cells migrate further than outer lateral cells. (A) A time-lapse sequence in which two IL cells were photoactivated, and then a second cluster of cells slightly further from the midline was photoactivated. Based on the typical number of cells in the invaginated epithelial tube, the cells labelled second are likely to be situated on the lateral side of the invaginated epithelial tube (see C′′). We define these cells as outer lateral (OL) cells. (B) Zero time-point. Reconstructed cross-sections through the dotted lines are shown in C and D. (C′,D′) The expected positions of these cells in the furrow at the time of panel B. (C′′,D′′) The expected positions in the invaginated tube following gastrulation. (E) Following spreading, the IL cluster has moved to a more dorsolateral position than the OL cluster (arrowheads).
Movie 1. Whole mesoderm time-lapse sequence. See Fig. 3. Each time point is a maximum projection of five confocal slices. All mesodermal cells over approximately three segments are photoactivated. Mesodermal cells go through the expected first (∼0:30:00) and second (∼1:20:00) divisions as they spread into a monolayer. Towards the end of the movie, some cells can be seen to undergo their third division at around 2 hours 30 minutes. Discontinuities in the movie are due to the z-limits being reset, so as to capture the increasingly three-dimensional extent of the mesoderm.
Movie 2. Outer cells migrate as a group over the ectoderm. See Fig. 4B,D. Two outer cells are tracked in the upper panel as they spread laterally. Lower panel shows reconstructed cross-sectional view through the marked cells.
Movie 3. Inner cells can move over outer cells. See Fig. 5. An outer cell, which initially is the most dorsolaterally placed cell, is tracked for 14 minutes. In the reconstructed cross-sectional view in the lower panel, inner cells can be seen moving over and past the marked cell, arriving at a more dorsolateral position.
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