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Fig. 5. Larval body wall muscle phenotypes of Poxm and l(1)sc
single and double mutants, and of Poxm mutants rescued by early
Poxm. (A-D) The somatic muscle patterns of
PoxmR361 embryos (A), PoxmR361 embryos
rescued by two copies of the um1-2-Poxm transgene (B), of
Df(1)sc19/Df(1)sc19 or Y (C),
and Df(1)sc19/Df(1)sc19 or Y;
PoxmR361 (D) embryos and of y w control embryos
(not shown) were analyzed at stage 16 under bright-field optics in a Zeiss
Axioplan 2 microscope after staining with anti-MHC antiserum, dissection along
the ventral midline, and removal of internal tissues. Each muscle plotted on
the abscissa was scored for absence (red), abnormality (yellow), duplication
(purple), or wild-type appearance (green) in each of 108 (A-C) or 168 (D)
hemisegments in abdominal segments A2-A7, and the resulting fractions were
plotted on the ordinate. Muscles of the y w control embryos were
usually normal in all 108 hemisegments scored, with the occasional absence,
duplication or abnormality of a single muscle. (E) The fractions of
normal muscles shown in A-D are plotted for Poxm mutants rescued by
early Poxm (purple), Poxm (red) and l(1)sc (yellow) single
mutants, and for l(1)sc; Poxm (green) double mutants,
whereas muscles are ordered along the abscissa with decreasing abnormality of
Poxm mutant phenotypes.
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