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Figure 5


Fig. 5. FOS-1 is expressed in the intermediate precursors and co-localizes with EGL-13 during the {pi} lineage. (A) fos-1 locus with isoform a, b, and putative c translational starts and origin of plastFOS-1c::YFP indicated. (B-F) Late L3 stage. (B) The AC (black arrowhead) is invading underlying primary vulval cells (black bracket). (D) Approximately 5 µm from B, FOS-1c is expressed in all dorsal and ventral uterine cells including the VU intermediate precursors (C, white arrowheads). (E) Three {pi} cells express egl-13::CFP (white bracket). (F) Merged image of D and E showing that FOS-1c co-localizes with egl-13 at specification. (G-J) Mid L4 stage. (H) FOS-1c is broadly expressed in numerous uterine cells. White arrows point to six {pi} cell daughters plus the AC. (I) {pi} descendants continue to express egl-13::CFP. (J) Merged image of H and I showing FOS-1c also co-localizes to the {pi} descendants. Asterisks mark non-specific fluorescence from the GFP co-transformation marker. Scale bar: 10 µm.





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