DEV007823 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. meis3 does not co-localize with the endodermal marker foxa1. Double fluorescent in situ hybridization at 24 hpf showing foxa1 in the endoderm (green) and meis3 (red) in non-overlapping domains. Top: merged color channels with nuclear staining in blue. foxa1 also labels the hypochord and the floor plate.

• Supplemental Figure S2 -

  Fig. S2. isl1 and meis3 knockdown reduces the exocrine tissue. Embryos were injected with isl1 or meis3 morpholino (MO) and the expression of the exocrine marker trypsin (try) was analyzed by in situ hybridization at 76 hpf. Ventral views, anterior to the left and left side to the top. (A) Control embryos injected with a control morpholino (meis3 MO with five mismatches or unrelated MO). (B) Representative embryo injected with isl1 MO out of the 88% injected embryos exhibiting defects (n=158) (top), and injected with meis3 MO out of the 95% embryos (n=203) (bottom).

• Supplemental Figure S3 -

  Fig. S3. erm is expressed in the pancreatic LPM before ventral pancreatic bud induction. erm (green) and isl1 (red) expression at 28 hpf. Two sections in the region of the prospective ventral pancreatic bud are shown. The yellow color results from superposition of strong isl1 and erm expression in the same cells.

• Supplemental Figure S4 -

  Fig. S4. fgfr2 is broadly expressed in both the mesoderm and the endoderm within the pancreatic region. The fgfr2 expression domain (green) during pancreas development includes several tissues including the pancreas, as revealed by pdx1 (red), and the lateral plate mesoderm, as shown at 28 hpf. Two sections in the region of the prospective ventral pancreatic bud are shown.