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Fig. 4. fgf24 is expressed in the pancreatic endoderm and in the
pancreatic LPM prior to and during ventral pancreatic bud formation.
(A) fgf24 (green) expression analysis by fluorescent
whole-mount in situ hybridization at 24 hpf with pdx1 probe (red). A
whole-mount ventral view (epifluorescence microscopy) is shown on the left
panel (anterior to the left and left side of the embryo up) with anterior (a)
and posterior (p) level of section. The transverse sections were analyzed by
confocal microscopy, through the anterior pancreatic domain (middle panel,
global view; right panel, close-up) and more posteriorly, through the dorsal
pancreatic bud (right panel, close-up). (B) Expression of
fgf24 (red) compared with isl1 (green) at 32 hpf (transverse
section, close-up in the right panel). Expression in the pancreatic LPM is
indicated by orange arrows. (C) fgf24 and isl
expression at 36 hpf. fgf24 expression appears as small red grains
owing to its weak expression. (D) fgf24 (green) and
ptf1a (red) expression at 32 hpf. The images of transverse sections
presented in B and C are flat stacking of several consecutive optical
sections. DB, dorsal pancreatic bud; FB, pectoral fin
bud.
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