DEV007138 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Sequence alignment of SMEDOLLOID-1 with other BMP1 family proteins. The SMEDOLLOID protein sequence was deduced from the full-length cDNA sequence. Alignment with Colloid from chicken (G.g.) and Tolloid from Drosophila (D.m.) is shown. Blue arrow indicates the region used to design the degenerate primer used during cloning. The astacin catalytic domain is indicated by the red line (above sequence). SMEDOLLOID-1 and Colloid share 63% identity in this region. Five CUB domains are indicated by green lines (above sequence) and the two EGF-like domains by yellow lines (above sequence).

• Supplemental Figure S2 -

  Fig. S2. Sequence alignment of SMEDSMAD4-1 with human (H.s.) SMAD4 protein. The SMEDSMAD4-1 protein sequence was deduced from the full-length cDNA sequence. The red line (above sequence) indicates the N-terminal MH1 domain and the blue line (above sequence) indicates the MH2 domain.

• Supplemental Figure S3 -

  Fig. S3. Sequence alignment of SMEDBMP4-1 with mouse (M.m.) BMP4 protein. The SMEDBMP4-1 protein sequence was deduced from the full-length cDNA sequence. The red line (above sequence) indicates the TGF-β domain.

• Supplemental Figure S4 -

  Fig. S4. BMP pathway components are required for lateral regeneration. Animals were amputated along the plane of bilateral symmetry to generate equal left and right animal halves. Anterior, left. Red arrows, side of regeneration. (A) Red line in diagram (to left) indicates site of amputation. Animals are pictured at 10 days of regeneration. smedolloid-1(RNAi) and smedsmad4-1(RNAi) animals went through one round of regeneration, and smedbmp4-1(RNAi) animals through two. 54/59 smedolloid-1, 60/66 smedsmad4-1 and 24/54 smedbmp4-1 RNAi animals were similar to the representatives shown. 53/60 control unc-22(RNAi) animals were normal. (B) H.1.3B labeling was performed with animals fixed 10 days after amputation. The smedolloid-1(RNAi) animal lacks large regions of lateral tissue; 10/12 animals were similar to the representative shown. Scale bars: 0.5 mm in A; 0.2 mm in B.