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Figure 1


Fig. 1. smedolloid-1 is expressed in small dorsally located cells and smedbmp4-1 is expressed in cells along the dorsal midline. (A) Schematic of a planarian depicting body regions and terminology used throughout this manuscript. Anterior, up. Dotted line indicates the dorsal midline. Solid lines indicate surgical amputations. (B,C) Dorsal (B) and Ventral (C) views of wild-type animals labeled with the smedolloid-1 riboprobe. An enlarged view of a head region is shown on the right in B. (D) A smedolloid-1(RNAi) animal showed no labeling in an in situ hybridization with a smedolloid-1 riboprobe. Scale bar: 0.1 mm. (E) Wild-type animals were labeled with the smedbmp4-1 riboprobe. px, pharynx. Arrowhead, smedbmp4-1 signal at the anterior end of the pharynx. Scale bar: 0.5 mm. (F) Section of a wild-type animal showing expression of smedbmp4-1 in sub-epidermal, dorsally localized cells. Following whole-mount in situ hybridization with a smedbmp4-1 riboprobe, animals were sectioned as previously described (Reddien et al., 2005b). In both panels, dorsal is to the right. Left panel, DAPI staining; right panel, Nomarski optics. E, epidermis. Scale bar: 10 µm. (G) A smedbmp4-1(RNAi) animal showed no labeling in in situ hybridizations with a smedbmp4-1 riboprobe. Anterior, left. Scale bar: 0.1 mm.





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