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Fig. 2. A slow transformation of adult form occurs following prolonged
perturbation of smedbmp4-1 signaling. Control
unc-22(RNAi) animals are shown on the left, or top, of each set of
images. (A) unc-22(RNAi) and smedsmad4-1(RNAi)
animals were pictured following ten dsRNA treatments and 83 total days of
treatment. smedbmp4-1(RNAi) and smedolloid-1(RNAi) animals
were pictured following seven dsRNA treatments and 65 days of total treatment.
smedolloid-1(RNAi) animals display dorsal tissue ruffling (white
arrow), and smedsmad4-1 and smedbmp4-1(RNAi) animals display
extra photoreceptors (yellow arrows). Anterior, left. Scale bars: 0.1 mm.
(B) Magnification of fixed animals shows the extra photoreceptors in
smedsmad4-1(RNAi) (126 days of RNAi) and smedbmp4-1(RNAi)
(104 days of RNAi) animals. Anterior, up. (C-E)
smedsmad4-1(RNAi) animals were fixed 126 days following initial dsRNA
exposure and smedbmp4-1(RNAi) animals were fixed 104 days following
initial dsRNA exposure. Anterior, left. Scale bars: 0.1 mm. (C,D) Animals were
labeled with antibodies that recognize the photoreceptor neurons (VC-1,
anti-Arrestin) and the cephalic ganglia (SYT, anti-Synaptotagmin). (D) Extra
ventral nerve cords (green, vnc2) were present in a smedsmad4-1(RNAi)
animal, located dorsal to the original nerve cords (red, vnc1). Animals were
imaged with Zeiss Apotome-based optical sectioning, and the two sets of nerve
cords false-colored. The regions shown are in the posterior of the animals.
(E) In situ hybridizations with the smedbmp4-1 riboprobe.
smedsmad4-1(RNAi) animals had no detectable smedbmp4-1
expression. (F) Animals were labeled with an antibody that recognizes
cilia (anti-acetylated tubulin). The dorsal surface of
smedsmad4-1(RNAi) animals display ventral-like cilia. The
smedbmp4-1(RNAi) animals were fixed 117 days following initial
exposure to dsRNA. Anterior, left. Scale bars: 0.05 mm.
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