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Fig. 2. CAM-1 CRD binds Wnts CWN-1, EGL-20 and MOM-2. Drosophila S2
cells expressing Neurotactin (Nrt)-HA-tagged C. elegans Wnts were
incubated with secreted CRDs of C. elegans Wnt receptors fused to
alkaline phosphatase (CRD-AP). (A) Levels of Nrt-HA-Wnt fusion proteins
(
130 kD) expressed by S2 cells were measured by anti-HA immunoblot. Wnts
are post-translationally modified and this might account for the detection of
multiple bands. Anti-GAPDH provided a loading control. (B) Amount of
CAM-1 CRD-AP retained by Nrt-HA-Wnt-expressing S2 cells. The assay was
performed in triplicate. As the untransfected sample appeared to contain
slightly fewer cells, we used cells expressing Nrt-CWN-2 (which expressed Wnt,
but did not bind CAM-1 CRD-AP) as a negative control for statistical analysis.
*, P<0.05, calculated using Fisher's exact test. Error
bars, s.e.m.
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