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Fig. 1. snail1b knockdown induced morphological defects at gastrulation
and segmentation stages. (A) Two different morpholino antisense
oligonucleotides were designed against adjacent sequences in the 5'
region of the snail1b cDNA. (B,D) Embryos at
mid-gastrulation and (C,E) at the 18-somite stage. Morpholino
injection (3 ng/embryo) produced defects in extension movements at early
embryonic stages that led to the shortening of the body axis at 30 hpf
(compare H with I). (F,G,J) This phenotype
was rescued by co-injection of snail1b mRNA.
(K,L,N,O,P,R) snail1a but
not snail1b was prominently expressed in the involuting and posterior
mesoderm. (M,Q) Lateral views highlight the expression of
snail1a in the posterior half of the embryo and snail1b in
the anterior. The dotted lines indicate the position of the margin and the
adaxial cells (P) and that of the prechordal plate (R). ac, adaxial cells;
ame, anterior axial mesendoderm; cm, condensing mesenchyme; pm, paraxial
mesoderm; tbm, tail bud mesenchyme.
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