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Fig. 7. Snail1a and Snail1b cooperate in the directed anterior migration of the
axial mesendoderm. (A-D) Morphological appearance of control
embryos (A), and of embryos injected with snail1a MOs (B), with
snail1b MOs (C) or with both (D). The expression of hgg1
(green) and snail1b (red) is superimposed. Note the abnormal position
and/or shape of the prechordal plate. The arrows mark the territory occupied
by the polster. (E-H) High-magnification confocal microscopy images of
the same embryos at the regions indicated with a red square in A-D. Red arrows
indicate the aberrant position of groups of snail1b-expressing cells,
and green arrows that of hgg1-expressing cells. (I-L) Diagrams
summarising the phenotypes observed in the snail1 morphants. (J) As
previously described, the injection of snail1a MOs altered the
posterior position of the prechordal plate (shown in green, with the extent
indicated by a double-headed arrow). (K) snail1b morphants developed
an elongated prechordal plate, which reached the normal anterior position. (L)
Co-injection of snail1b and snail1a MOs produced a compound
phenotype in the prechordal plate, which adopted an elongated shape and was
situated at an aberrant posterior position. Black arrowheads indicate the
anterior and posterior limits of the embryo. The double-headed arrows on the
outside of the embryos mark the distance between the embryo limits.
(M-P) hgg1 in situ hybridisation and DAPI (nuclei) staining
show the cell density in the regions indicated with a dotted blue square in
I-L. All embryos in this figure are at tail bud stages.
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