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Fig. 7. Reduced proliferation and early progenitor cell cycle exit in
cD2-/- MGE. All sections, 4 µm. (A) Fewer
nuclei label with the M-phase marker PH3 in the VZ and SVZ of the
cD2-/- MGE than in WT. (B-D) Fewer nuclei are
labeled by a 1-hour BrdU pulse in cD2-/- (C) than WT (D)
MGE. The proportion of total (DAPI) nuclei labeled by anti-BrdU is
significantly reduced in the SVZ. (E-G) Dual-immunolabeling with BrdU
and Ki67 (to label S-phase through M-phase cells) shows the total numbers of
nuclei labeled by a 24-hour BrdU pulse is slightly reduced in
cD2-/- MGE, but numbers of BrdU+ nuclei not co-labeled
with Ki67+ are increased in the MGE of cD2 nulls (F), as compared
with WT (E). Quantification of dual (P fraction) and single (Q fraction; BrdU
alone) labeled cells indicates a decrease in the proliferating, P fraction and
a corresponding increase in the quiescent, Q fraction in
cD2-/- MGE (G), suggesting progenitors are prematurely
depleted in cD2-/- mice. *, P<0.05;
**, P<0.01; ***, P<0.003.
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