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Fig. 2. Loss and gain of Lrp6 function in explants affects convergent-extension
movements. (A) lrp6 mRNA (LRP6; 1 ng) or Lrp6MO (LRP6MO;
40 ng) injections block activin-mediated animal cap elongation, in a similar
manner to injection of Wnt11 (400 pg) control. By contrast, injection of
ß-catenin at levels that cause complete axis duplication (50 pg) has no
effect. (B) RT-PCR analysis shows that mesendodermal markers (Xbra,
Chordin, Goosecoid, Sizzled, Endodermin) are induced by activin in animal caps
and their expression is unaffected by injections of Lrp6MO or lrp6
mRNA. Note induction of Xbra and Chordin after injections of similar amounts
of dsh mRNA (1 ng). Loading control: ODC (ornithine decarboxylase).
(C) Keller sandwiches elongate when DMZ explants are cultured in
apposition. Keller sandwiches injected with Lrp6MO (40 ng) or lrp6
mRNA (1 ng) show impaired elongation (compared to control uninjected or
ß-catenin expressing explants) and resemble those expressing Xdd (2 ng).
ANE, anterior neural ectoderm; PNE, posterior neural ectoderm; IM, involuting
mesoderm. Student's t-test was used for statistical analysis. Error
bars indicate standard deviation. Asterisks mark differences that are
statistically significant from control (P<0.01). Numbers of
explants scored are indicated in parentheses.
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