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Fig. 4. Lrp6 localizes in a polarized distribution in cells that undergo
convergent extension and is adjacent to actin-rich regions in cellular
processes. (A) Animal cap cells show uniform distribution of
injected VSVG-lrp6 (VSVG-LRP6; 2 ng). In Activin-treated caps the
staining of VSVG-Lrp6 is polarized. (B) DMZ explants from
VSVG-Lrp6-injected embryos show preferential localization of Lrp6 at the
posterior cell edge. High magnification views of cells in the DMZ are shown in
the bottom panels. Injection of myristoylated, plasma membrane localized GFP
(mGFP; 500 pg) or anti-actin staining was performed as controls for animal cap
and DMZ experiments, respectively. Explants are outlined with solid lines to
make their borders readily identifiable. (C) Lrp6 is adjacent to
actin-rich regions of cellular extensions. DMZ cells injected with VSVG-Lrp6
were dissociated, plated on fibronectin, immediately fixed, and stained with
anti-VSVG (green) and phalloidin (red). Scale bars: animal caps, 30 µm; DMZ
(40x), 200 µm; high-magnification DMZ (75x), 50 µm;
dissociated cells (200x), 20 µm; high-magnification dissociated cells
(350x), 5 µm.
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