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Figure 1


Fig. 1. Heparanase is expressed in mouse SMGs throughout development, mainly in the mesenchyme, and colocalizes with perlecan in the epithelial basement membrane. (A) RT-PCR analysis of heparanase, Fgf10, and perlecan were compared at various developmental stages. Gene expression was normalized to 29S and is expressed relative to gene expression at E12. Data were obtained from triplicate experiments, repeated three times, and are mean±s.d. (B) Relative abundance of gene expression comparing E13 epithelium with mesenchyme. cDNA were prepared from E13 epithelium separated from mesenchyme. Gene expression was normalized to 29S. (C) Immunolocalization of heparanase and perlecan in E13 SMGs cultured for 36 hours. Heparanase (green) localizes in the mesenchyme and colocalizes with perlecan (red) in the basement membrane. The images are of a single 2 µm confocal section. Nuclei stained with SYBR-green (blue).





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