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Figure 7


Fig. 7. FGFR2b and the FGF10-FGFR2b complex colocalize with endogenous SMG perlecan HS, and the binding is decreased by heparanase treatment. A whole-mount ligand and carbohydrate engagement (LACE) assay using E13 SMGs shows that FGFR2b (A) and the FGF10-FGFR2b complex (B) colocalize with perlecan in the basement membrane. Increased binding was detected with the complex compared to the receptor alone. There was decreased binding of both FGFR2b and the FGF10-FGFR2b complex after heparanase or heparitinase treatment. In addition, the FGF10-FGFR2b complex also colocalizes with syndecan 1 in the epithelium (C), but also binds other epithelial HSPGs, and the staining was not decreased by pretreatment with either heparanase or heparitinase (data not shown). Images are single confocal sections. Scale bar: 20 µm in A and B; 10 µm in C.





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