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Fig. 6. Ectopic expression of HyBra1 and HyBra2 in response to
alsterpaullone treatment. Animals were exposed to 5 µM alsterpaullone
(AP) for 24 hours and subsequently transferred to Hydra media (HM) for 15
hours and 40 hours respectively. HyBra1 (A-D), HyBra2
(E-H) and Wnt (I-L) are affected by the GSK-3 inhibitor
alsterpaullone. Ectopic HyBra1 and HyBra2 expression starts
24 hours after AP treatment in single cells (B,F, high-magnification inset)
whereas normal hypostome expression declines rapidly and is shifted to the
tentacle bases (compare A,B with E,F); elevated levels of expression are seen
over the uniform endodermal expression (compare A,E with B,F). In later
stages, the single cell expression in the body column begins to cluster
(arrows in C and G) and subsequently peaks in a stochastic pattern of spots of
10-15 epithelial cells (arrows in G and H). HyWnt hypostome
expression is lost 15 hours after completion of AP treatment and clusters
along the body column in ectopic expression domains are narrower than those of
HyBra1 and HyBra2.
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