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Figure 7


Fig. 7. Distinct morphological phenotypes induced in Xenopus animal caps by Hydra Brachyury proteins. (A) Schematic view of the microinjected constructs (AD, activation domain; C-T, C-terminal domain). (B) Sketch of the animal cap experiment (st, stage). (C-E) Control specimens: (C) wt sibling embryo at NF36, (D) uninjected and (E) GFP-injected animal caps with default epidermal morphology. Animal caps injected with Xbra (F), HyBra1 (G) or H2TXA (I) with elongated morphology, indicating mesoderm formation. (H) HyBra2-injected animal cap with two darkly pigmented cement gland areas. (J) XTH2A-injected explant with single cement gland area. (K) Statistical overview of morphological characteristics [uninduced, elongated and cement gland celltype-specific (cg cells)] induced by different Brachyury paralogues (3-10 independent injections). RNA doses were titrated from 0.1 to 1.0 ng/embryo, resulting in consistent, qualitatively different phenotypes represented by the displayed specimen.





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