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Fig. 5. Cdx2 and Nanog variability are not correlated. (A) Cdx2 and
Nanog at defined stages of development. For every embryo the counterstained
DNA is shown in the row below. White and green arrowheads indicate nuclei of
interest of inside and outside cells, respectively. Actin (red) marks the cell
membranes. Asterisks indicate blastomeres with only minimal contact to the
outside. All images are single optical sections. Scale bar: 20 µm.
(B) Scatter plots showing the normalized mean fluorescence intensity of
Cdx2 relative to Nanog in individual blastomeres. Each dot represents one
blastomere; different colors represent different embryos. Red lines mark the
background fluorescence level.
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