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Fig. 3. In Drosophila Dorsal is highly mobile and shuttles from nucleus
to cytoplasm in both ventral and dorsal nuclei. (A) A fluorescence
recovery after photobleaching (FRAP) reveals that Dorsal-GFP is highly mobile
within the nucleus. A bleached area (red circle) before and after a 3 second
photobleach, showing extensive and uniform loss of nuclear fluorescence. This
indicates high mobility of Dorsal-GFP within nuclei. However, the extensive
fluorescence recovery by 154 seconds postbleach shows that nuclear Dorsal-GFP
must exchange with the cytoplasmic pool and thus undergoes nucleocytoplasmic
shuttling. (B) Dorsal is present in extreme dorsal nuclei and shuttles
from nucleus to cytoplasm. An identical FRAP conducted on a nucleus located on
the extreme dorsal side, showing both initial loss and subsequent recovery of
nuclear fluorescence over time. (C) Quantification of the data from the
ventral nuclear FRAP. To compesate for any change in nuclear levels over time,
internal nuclear fluorescence intensity was normalized to that of a ventral
nucleus at the same relative DV position. (D) Quantification of the
data from the dorsal nuclear FRAP. As in C, internal nuclear fluorescence
intensity normalized to a nearby (dorsal) nucleus at the same relative DV
position. Bleach boxes in both experiments are shown in red.
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