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Figure 4


Fig. 4. Dorsal is exported from nuclei by a CRM1-mediated process and has a carboxyterminal LRNES. (A) A saggital confocal section of a wild-type Drosophila embryo, showing the normal polarized nuclear Dorsal distribution. (B) A saggital confocal section of an embryo treated with LMB during nuclear cycle 14, showing Dorsal protein within dorsal nuclei, which normally do not accumulate Dorsal. (C) A dorsal view of embryo at the beginning of gastrulation. (D) A dorsal view of an LMB-treated embryo, showing perdurance of high levels of nuclear Dorsal during gastrulation. (E) Anti-Twist staining of an LMB-treated embryo, showing expansion of mesodermal cell fates. (F) Putative LRNESs within the carboxyterminus of Dorsal. (G) Schematic of tandem GFP constructs used in an in vivo assay for nuclear export. (H) Localization of Tandem GFP driven by nanos-GAL4. (I) Localization of tandem GFP with Dorsal's carboxyterminal 44 amino acids driven by nanos-GAL4. (J) Alignment of Putative LRNES sequences at the carboxyterminus with other Drosophila NF-{kappa}B proteins and several mammalian LRNESs.





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