QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Transient sens expression rescues inner photoreceptor specification. (A) Coronal section of 55% pupal and 70% pupal heads from sca^109.68-GAL4, UAS-nuclear GFP (sca>nGFP) Drosophila. All PRs are marked with Elav (purple). GFP (green) is present in R8 cells at 55% APF, but not after 70% APF. (B) Summary of sens expression in full LOF and late LOF experiments. (C-E) Adult retinal sections stained for Salm (green); dorsal left, distal up. (C) UAS-sens/+, sens^E1/TM6B: the R7 and R8 layers of Salm-positive nuclei are labeled. (D) sens^E1 full LOF eyes (see Materials and methods) rarely develop IPRs (arrowhead). (E) sens^E1 mutant eyes with sca^109.68>sens (sens late LOF) develop two Salm-positive layers of IPRs (arrows). (F-H'') Adult cryosections of control and sens^E2.1 late LOF retinas stained for Salm (green, F-H,F'-H'), Pros (purple, F'-H'), or Sens (white, F''-H''). F-F'', G-G'', and H-H'' represent eyes co-stained for all three factors. Pros stains distal Salm-positive cells in both control and sens^E2.1 late LOF retinas, and the majority of Salm-positive cells in sens^E2.1 full LOF retinas. Sens is expressed in R8 cells only in control retinas (F'').

Return to article