DEV005942 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Effect of LRP6 knock-down on development of the dorsal fin. (A) Control embryos. (B) Phenotypes of embryos injected with 1.5-2 ng LRP6 MO. Note the reduction of the dorsal fin (arrows) in embryos of otherwise normal morphology.

• Supplemental Figure S2 -

  Fig. S2. Krm constructs in Wnt reporter gene assay. Transfected DNAs: 24 ng flag-hLRP6; 1 ng mesd; 5 ng all krm constructs. Co, empty pCS2 vector. Note that the C-terminally truncated mKrm1 (mKrm1ΔC) has reduced activity compared with wild-type Krm1. Xenopus Krm2 promotes Wnt signaling, whereas mouse Krm2 consistently does not. This is probably due to a non-specific inhibition of downstream Wnt components by mKrm2. Right panel: all krm constructs are expressed at a similar level.