DEV009159 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Specificity test of anti-AGO1 antibody. (A) Ovaries dissected from w118 flies were stained with anti-AGO1 antibodies (red). (B) Ovaries dissected from w, hs-flp; FRTG13, Ago1¹⁴/FRTG13, ubi-GFP treated by heat shock were stained with anti-GFP (green) and anti-AGO1 (red) antibodies. An Ago1¹⁴ cysts clone marked by a lack of GFP expression is indicated by an arrow. Scale bar: 10 μm.

• Supplemental Figure S2 -

  Fig. S2. Loss of GSC in Ago1 mutant is not due to cell death. Ovaries from hs-Flp/+;FRTG13, Ago1^k08121/FRTG13, Ubi-gfp flies treated by heat-shock induction were labeled by TUNEL (green) (A) and stained with anti-GFP antibody (red) (B), showing that Ago1- GSC clones (indicated by an arrow in the merged image) (C) were not apoptotic. As a control, hs-Flp/+;FRTG13, Ago1^k08121/FRTG13, Ubi-gfp flies under the same treatment were labeled by TUNEL (green) (D) and stained with anti-GFP antibody (red) (E); a dying cyst is indicated by an arrowhead in the merged image (F). This result suggests that Ago1 is required for GSC self-renewal, rather than survival. Scale bar: 10 ?m.

• Supplemental Figure S3 -

  Fig. S3. An image from the right part of Fig. 1C, with spliting of the channels.