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Fig. 4. Two components of the miRNA pathway, AGO1 and Loqs, are not required for
bam silencing in Drosophila. Ovaries dissected from flies
{hs-flp; frtG13, Ago1k08121/frtG13, ubi-gfp} with
heat-shock treatment were stained with anti-BamC (red). Two wild-type GSCs
that are GFP-positive are negative for BamC (indicated by an arrow)
(A), and a negatively marked GFP GSC, indicated by an arrow, was also
BamC-negative (B). Ovaries dissected from P{bamP-GFP}
(C), loqs; P{bamP-GFP} (D), bam, P{bamP-GFP}
(E), loqs; bam, P{bamP-GFP} (F) were stained with
anti-GFP (green) and anti-Hts (red) antibodies. GSCs with negative GFP
expression are indicated by arrows. Scale bar: 10 µm.
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