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Fig. 3. Cell-row boundary smoothing and cell intercalation prior to
invagination. See Movies 1 and 2 in the supplementary material. (A)
GFP-Moesin. Intercalating cells are shown in the same color. Shrinking cell
boundaries and newly formed cell boundaries are indicated by blue and red
arrows, respectively. The position of the tracheal pit is indicated by a red
asterisk. Examples of cellular arc formation are indicated by orange and green
lines. (B) Analyses of the cell displacement angle. Mean±s.e.m.
are shown. Asterisk indicates significant (P<0.05) deviation from
random orientation (average 45°). See text for details. (C-E)
Transient accumulation of myosin-GFP at the cell-cell interface. (C)
Continuous rows of myosin-GFP-enriched cell-row boundaries are indicated by
arrows. Cell boundaries at the segment border and dorsal ectodermal margin
(green and orange arrows, respectively) stably accumulated myosin-GFP.
Myosin-GFP accumulation in the arc-like cell-row boundaries surrounding the
invagination site was transient and appeared as temporary waves (blue arrows,
see also Movie 2 in the supplementary material). (D) Cell boundary tracing.
Each arc-like boundary was formed by the joining of zig-zagging and
discontinuous boundaries, which changed with the arrangement of the cells
relative to one another (blue, green and magenta lines). The boundary marked
with blue lines became discontinuous before entering the tracheal pit. The
orange lines indicate cell boundaries in dorsal ectoderm, which were more
stable. Red asterisk indicates the invagination site. (E) High accumulation of
myosin-GFP in shrinking cell boundaries. For explanation of colored areas, see
A. Scale bars: 10 µm.
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