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Fig. 2. aPKC regulates subcellular localization of PAR1 in Xenopus
ectoderm. (A,B) aPKC inhibits cortical localization of PAR1.
(A) PAR1 is predominantly localized to the basolateral cortex of superficial
ectoderm cells (arrows). (B) Overexpression of aPKC-CAAX mislocalizes PAR1 to
the cytoplasm (arrowheads). (C,D) The apical localization of
T560A, the non-phosphorylatable PAR1 mutant (C') is not affected by aPKC-CAAX
(D). At higher doses of injected RNA, T560A was distributed all around the
cell cortex, both apically and basolaterally (C). The distribution of
coinjected membrane-associated aPKC-CAAX is shown in B' and D', the increased
apical staining is due to high amounts of endogenous aPKC detected by
anti-aPKC antibody. B" and D", merged images. Embryo injections
were as described in Fig. 1.
Frozen sections of stage 10.5-11 embryonic ectoderm were stained with anti-Myc
(green) to detect PAR1 (B,D) and anti-aPKC antibodies (B' and D', red). At
least 15 embryos per group were examined and representative sections of three
independent experiments are shown.
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