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Fig. 5. aPKC functions upstream of PAR1 to specify ectodermal cell fates.
Embryos were injected with RNAs or MO as described in
Fig. 1. Ciliated cells were
detected at stages 14-16 by in situ hybridization with the 
-tubulin
probe. (A-C) T560A reverses the inhibitory effect of aPKC-CAAX on
ciliated cell differentiation. Two sides of the same embryo are shown in B and
C. (D-F) PAR1B MO (F) suppresses aPKC-N-mediated expansion of ciliated
cells. The injected and uninjected sides of the same embryo are shown in D and
E, respectively. (G-J) Quantification of the data shown in A-C (G,H)
and D-F (I,J). Numbers of embryos per group are shown above bars. (G,I)
Frequencies of embryos showing visible phenotypic changes. (H,J) Mean numbers
of -tubulin-positive cells per section±s.d. are shown. Sections
of at least three representative embryos per group were analyzed.
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