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Fig. 8 . PAR1 synergizes with XDelta-1 to induce ciliated cell
differentiation and inhibits the Notch target ESR6e. Four- to
eight-cell embryos were unilaterally injected with the indicated RNAs and
lacZ RNA as a lineage tracer (light blue staining) and subjected to
in situ hybridization with the ESR6e (A,B) or 
-tubulin (E-L)
probes. (A,B) Superficial staining for ESR6e is
unaffected in cross-sections of lacZ RNA-injected control embryos (A,
100 pg), but is inhibited in PAR1 RNA-injected embryos (B, right side,
arrowhead, 250 pg). (C) Basolateral localization of XDelta-1 in
Xenopus ectoderm. (D) XDelta-1 is detected in multiple
cytoplasmic vesicles (arrowheads) in the presence of PAR1. (E)
Uninjected embryo. (F,G) XDelta-1 RNA alone (F) or low dose of
PAR1 RNA (G) do not significantly alter the number of ciliated cells.
(H) The synergistic effect of coinjected PAR1 and XDelta-1 RNAs on
ciliated cell development. (I,J) PAR1 does not influence the
activity of a dominant intracellular inhibitor of the Notch pathway, dnRBP/j,
which can stimulate ciliated cell development. (K) Notch-ICD suppresses
ciliated cell differentiation. (L) PAR1 does not alter Notch-ICD
activity. (M-O) Quantification of the effects shown in E-J, presented
as numbers of ciliated cells per section (M,N) and frequency of embryos with
increased -tubulin staining (O). Numbers of examined embryos are shown
above bars. The data are representative of three independent experiments.
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