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Figure 1


Fig. 1. Histological and fate map analysis of chick tail development. (A) A schematic illustration of chick caudal embryogenesis. cl, cloaca; cm, cloacal membrane; hg, hind gut; ps, primitive streak, tb, tailbud; ver, ventral ectodermal ridge. (B) The tailbud of chick embryos at HH stage 18. The white line in B indicates the approximate level of the section shown in C. Scale bar: 400 µm. (C) Transverse section of the chick tailbud. no, notochord; nt, neural tube; psm, presomatic mesoderm; tvm, tail ventral mesoderm. Bar: 100 µm. (D) High magnification of the box in C, showing the chick VER and the adjacent region. Scale bar: 50 µm. (E) Immunostaining with laminin and E-cadherin of the chick VER. Bar: 50 µm. (F) Fate mapping of the tailbud ectoderm including the chick VER. The ectoderm was labeled with DiI at HH stage 16 and 20 (left panels). Middle and right panels show distribution of labeling after 24 hours. The white line in the middle panels indicates the position of the sections shown on the right. hl, hind limb. Scale bars: whole mounts, 800 µm, 400 µm; sections, 100 µm.





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