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Fig. 3. Nog overexpression in the tailbud using sonoporation.
(A) A schematic drawing of the sonoporation procedure. (B-E) GFP
expression after Sonoporation. The white lines in B and D indicate the
position of the sections in C and E. Scale bars: 400 µm (B,D); 50 µm
(C,E). (F) Morphology of the tailbud of control and
Nog-overexpressing chick embryos. Scale bar: 200 µm. (G)
pCAβ-mNog-IRES-GFP or pCAβ-IRES-GFP were transduced into
the caudal region of the chick tailbud. The surface ectoderm of the tailbud
was labeled with DiI after sonoporation. The distribution of DiI-labeled cells
in control and Nog-overexpressing embryos was observed after 24
hours. Scale bars: 200 µm (left); 100 µm (middle and right).
(H,I) Hematoxylin and Eosin staining of control and
Nog-overexpressing embryos. (J,K) GFP expression in
control and Nog-overexpressing embryos. (L,M)
Immunostaining of pSmad (red) and E-cadherin (green) in control and
Nog-overexpressing embryos. (L',M')
High-magnification view of boxed area in L and M. (N,O)
Immunostaining of E-cadherin (green) and laminin (red) in control and
Nog-overexpressing embryos. (P,Q) cSlug
expression in control and Nog-overexpressing embryos. Scale bar: 50
µm.
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