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Figure 6


Fig. 6. A histological analysis of the mouse VER and kinetics of mNog expression pattern and basal membrane degradation during mouse tail development. (A) The tailbud of a mouse embryo at E10.5. The white line indicates the approximate level of the section shown in B. Scale bar: 400 µm. (B) Transverse section of the mouse tailbud. No, notochord; Nt, neural tube; PSM, presomatic mesoderm; Tg, tail gut; TVM, tail ventral mesoderm; VER, ventral ectodermal ridge. Scale bar: 100 µm. (C) High magnification of the boxed region in B. Scale bar: 50 µm. (D) Immunostaining with laminin (red) and E-cadherin (green) of the mouse VER. Scale bar: 50 µm. (E) The kinetics of mNog expression pattern and basal membrane degradation. An arrow indicates the primitive groove. Scale bar: 50 µm. Note the low level mNog expression in the TVM at E9.0 (red arrowhead) and the associated basal membrane degradation underlying the ectoderm derived from the late primitive streak (white arrowheads). Prominent mNog expression was localized in the TVM and basal membrane was formed underlying the mouse VER until E10.0-10.5. Scale bars: black, 50 µm; white, 10 µm.





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