DEV012047 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Genomic PCR analysis of total E9.5 embryo DNA shows efficient and complete excision of the conditional allele in Sox2-Cre:BEH/flox embryos.

• Supplemental Figure S2 -

  Fig. S2. Blimp1 inactivation causes loss of posterior structures including muscles and connective tissue, without affecting fore limb outgrowth or dorsal ventral polarity. External appearance of mutant (A) and wild-type (B) limbs at E18.5. The altered morphology of the mutant limbs reflects selective loss of the ulna (C) and digits 1 and 2 (D). D-V polarity of the muscles and tendons is unaffected in the mutant (D) compared with the wild type (E).

• Supplemental Figure S3 -

  Fig. S3. Fgf8 and Tbx3 WISH reveals progressive loss of the posterior AER and underlying mesenchyme in mutant limb buds. Red lines and asterisks indicate AER and mesenchyme tissue present in the wild type that is selectively lost in the mutants.

• Supplemental Figure S4 -

  Fig. S4. Ki67 and phospho-histone 3 staining of serially sectioned matched tissue. A similar mitotic labelling index is observed in independent wild-type and mutant forelimb bud samples.

• Supplemental Figure S5 -

  Fig. S5. Shh and Gli1 expression is transiently induced but not maintained in E13.0 mutant DP progenitor cells.