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Fig. 2. Blimp1-deficient forelimbs fail to maintain the ZPA.
Comparison of wild-type
(A,C,E,G,I,K,M,O,Q)
and mutant
(B,D,F,H,J,L,N,P,R)
forelimb (FL) and hindlimb (HL) buds. (A-C) Activation of Shh, a
marker for the ZPA, proceeds normally at E10.5 in Blimp-deficient
limb buds, but is markedly reduced in the forelimb bud (B). By E11.5,
Shh expression is confined to a small patch of distal mesenchyme in
mutant forelimbs (D). (H) Blimp1 mutant limbs express Fgf4
in the forming AER at E10.5. By contrast, by E12.5 Fgf8 is absent
from the mutant posterior proximal limb (J), owing to loss of the AER and
underlying mesenchyme. Lmx1 expression shows normal D-V patterning in
the forelimb (K,L). Tbx2 (N), and Gli1 (P) in
Blimp1-deficient forelimb buds highlight the rapid loss of posterior
tissue. Grem1 (Gremlin) is expressed in mutant limb buds albeit at
lower levels (R).
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