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Figure 2


Fig. 2. Roles of fusion proteins in actin remodeling. Lateral views of stage 14 rp298-lacZ embryos stained with phalloidin to label F-actin (red) and antibody against β-galactosidase to label FCs/myotubes (blue), except E, which is stained with an antibody against Slouch to label a subset of FCs/myotubes (blue). Actin foci are indicated by arrowheads. Scale bar: 5 µm. One optical slice is shown for each mutant. For details of focus size determination, see Materials and methods and Fig. S2 in the supplementary material. (A) Wild type. (B,C) Class 1: no, or few foci. (B) snsXB3 embryo. (C) rolsT627 embryo. A focus of wild-type size is occasionally seen (arrowhead). (D) Class 2: wild-type actin foci: lonerT1032 embryo. (E-H) Class 3: enlarged actin foci. (E) Rac1J11, Rac2{Delta}, mtl{Delta} embryo. (F) ketteJ4-48 embryo. (G) blow1 embryo. (H) mbcC1 embryo.





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