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Fig. 2. TrkB and TrkC are essential for proliferation of cultured murine
embryonic cortical precursor cells. (A) Immunostaining for EGFP
(green, GFP) and Ki67 (red) for precursor cells transfected with EGFP and
empty vector (control), dnTrkB, dnTrkC or both (dnTrkB/C) and cultured for 2
days. Cells were counterstained with Hoechst 33258 (blue). Arrows denote
double-labeled cells. (B) Quantification of the percentage of
transfected, Ki67-positive cells in experiments similar to A. Two experiments
of four are shown. (C,D) Quantification of the percentage of
apoptotic cells in experiments similar to A, except that cells were cultured
with or without 100 µM ZVAD-FMK. Apoptosis was assessed by (C) analysis of
condensed, fragmented nuclei and by (D) immunostaining for EGFP and cleaved
caspase 3. In both panels, one experiment of two is shown. (E)
Quantification of the percentage of transfected, Ki67-positive cells in
experiments similar to A, except that cells were cultured with or without 100
µM ZVAD-FMK for 2 days. Error bars indicate s.e.m. Scale bar: 100 µm.
*P<0.05, **P<0.01,
***P<0.001 relative to control-transfected
cultures.
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