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Fig. 8. Inhibition of Trk signaling in embryonic cortical precursors leads to postnatal perturbations in cortical neurons in mouse. Cortices were electroporated with EGFP and the empty vector (control), dnTrkB, dnTrkC or both, and analyzed at P3. (A) Confocal micrographs of coronal sections through cortices immunostained for EGFP (GFP, green) and NeuN (red). The right panels show the merges. Arrows indicate double-labeled cells. (B,C) Quantification of micrographs similar to A for percentage of (B) EGFP, NeuN or (C) EGFP, HuD-positive cells within the cortical layers. n=five, five sections/brain. (D) Photomicrographs of coronal sections immunostained for EGFP (green) and counterstained with Hoechst (blue). Cortical layers are denoted on the left, and regions defined as the upper and lower parts of layers II/III are demarcated by the white lines. (E) Quantification of the percentage of EGFP-positive cells in the upper (white columns) and in the lower (black column) parts of layers II/III in sections similar to (D). n=five, five sections/brain. (F) Photomicrographs of coronal sections immunostained for EGFP (green) and GAD67 (red) and counterstained for Hoechst (blue). Shown are EGFP and Hoechst (left) or EGFP and GAD67 (middle). The right panel is a higher-magnification view showing a rare, double-labeled cell (arrow), and several EGFP-positive, GAD67-negative cells (arrowhead). Error bars indicate s.e.m. Scale bars: 100 µm in A; 200 µm in D; 50 µm in F. ^***P<0.001 relative to control-transfected sections.

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