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Fig. 3. Ngn1 expression decreases over time in the prospective
utricle. (A) E14.5 utricular macula schematized in the same
orientation as the scatterplots in B and E-E''. A cross-section of the
macula along the dotted line is represented at the bottom. The macula (region
of myosin VIIa staining) is in gray. The active neurogenic region (Ng, region
of Ngn1-GFP staining) is white. ut m-lc, non-sensory tissue between the
utricular macula and lateral crista; ut m-sac m, non-sensory tissue between
the utricular macula and saccular macula. (B) Distribution of
GFP+ cells (blue crosses, cyan in image) from three E14.5 Ngn1-GFP
BAC transgenic utricles, plotted on a normalized scale, defines the region of
active neurogenesis. Gray area represents an averaged macular area (myosin
VIIa +); the yellow area is non-sensory epithelium between the
utricular macula and lateral crista. White arrow marks the lateral extent of
the macula. (C) Number of Ngn1 derivatives in the Ngn1-CreER;Z/EG
utricle versus time of first tamoxifen feeding for embryos sacrificed at
E14.5. Each point is based on four or more ears from two or more litters.
(D) Sections through utricular maculae, representing the change in the
spatial distribution of Ngn1 derivatives (green) with different tamoxifen
regimens. Downturned brackets indicate overlap between Ngn1 derivatives and
myosin VIIa + hair cells. Upturned brackets indicate the region of
active neurogenesis. White arrows indicate the lateral extent of the macula.
Asterisk shows an Ngn1 derivative in non-sensory tissue between the utricular
macula and lateral crista. (E-E''') Spatial distributions of Ngn1
derivatives from E14.5 utricles exposed to different tamoxifen administration
regimens, as indicated at the top of each plot. (E) n=4 utricles;
(E') n=6 utricles; (E'') n=14 utricles. Ngn1
derivative cell types and regions of the plot are coded as shown in
E'''.
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