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Figure 5


Fig. 5. ARF6 endosomes at the Pav central spindle during cytokinesis. (A,B) Fixed primary spermatocytes. (A,B) ARF6HA (red) and Pav (blue) immunostaining and Rab-GFP [green; Rab4 (A) and Rab11 (B)]. ARF6HA colocalizes with GFP-Rab4 (A) and GFP-Rab11 (B) at the central spindle (arrowheads). (C) Colocalization frequency of GFP-Rab4 (n=17 cells) and GFP-Rab11 (n=13 cells) with ARF6HA at the central spindle during cytokinesis (grey) or elsewhere in the cell (blue). Error bars represent s.e.m. (D) Primary spermatocyte initiating furrow. ARF6HA (red) is already partially localized to the central spindle (arrowheads), labelled with Pav-GFP (green). DAPI labels chromosomes (blue). Scale bars: 5 µm.





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