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Fig. 4. perd and Grip interact specifically in a novel RNAi
interaction assay. (A) Injection of Grip dsRNA at high
concentration phenocopies a loss-of-function mutation. (B-D)
Representative embryos injected with perd dsRNA (B) or Grip
dsRNA (C) at concentrations titrated to give minimal effect, and the stronger
effect observed when they are combined (D). (E) Quantification of
pooled data from four independent experiments, comparing the frequency with
which effects are seen from each dsRNA individually or from both together,
along with the distribution predicted if effects were additive (see Materials
and methods for details). n=the number of informative embryos scored
for each condition. The probability of obtaining the observed frequencies from
the additive distribution was <10-12 (
2 test).
(F,G) In identical experiments, mib2 dsRNA does not
exhibit synergy with perd or Grip dsRNA, despite causing
detachment and rounding of a similar subset of muscles when injected at high
concentration.
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